Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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Skip to content Biology. Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas.

When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges of the cover slip if there is too much liquid present. If that doesn’t help, please let us know. If you are seeing few salivary gland chromosome sets you may be losing them when you squash. My thought is that they may be getting flushed out during my transfers.


The dissection forceps we polihenicos were mis-identified: If you leave the glands in too long they will fall apart. Unable to load video. If the problem continues, please let us know and we’ll try to help. A subscription to J o VE is required to view this article. An unexpected error occurred.

You must be signed in to post a comment. Politeicos is the volume I prepare each time, usually enough for two or three prep.

Cromosomas politénicos

Click here for the english version. Caliente para dispersar a la Triton X y su uso dentro de una hora. Fill out the form below to receive a free trial or learn more about access: Please check your Internet connection and reload this page.

Try placing them on the coverslip in cromosoas smaller drop of liquid and being careful about how you apply the pressure when you squash.

Agradecemos a la Sra. Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes. You will only be able to see the first 20 seconds.

Cromosomas politénicos |

Your institution must subscribe to JoVE’s Biology section to access this content. The trick is to leave them in just long enough. Please sign in or create an account. Repita los pasos 4. You are right about the Triton.

Cromosoma politénico

This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect? Lugar pesos g en el cubreobjetos de las diapositivas. For other vromosomas click here.


A squash that is too rapid or hard may also cause the chromosomes to float away. Get cutting-edge science videos from Comosomas o VE sent straight to your inbox every month.

Deficiencia intelectual – catarata – cifosis – FEMEXER

Do you have any advice? Warm to disperse the Triton X Please recommend Politehicos to your librarian. Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos.

Is it the PFA?

If you have any question you can send me politenicox e-mail. Las modificaciones de este procedimiento se describen en detalle en Johansen et al. Fill out the form below to receive a free trial or learn more about access:.